目的 研究烧伤科多重耐药肺炎克雷伯菌抗菌制剂外排泵基因的流行情况.方法 收集从烧伤患者分离的多重耐药肺炎克雷伯菌20株,采用kb法检测其对14种常用抗菌药物的敏感性.用聚合酶链反应(pcr)检测抗菌制剂外排泵基因oqxa、smrkpn、qace、teha、mdfa和qaceΔlsul1.结果 20株多重耐药肺炎克雷伯菌除了对亚胺培南具有较高敏感性外,对其他抗菌药物的敏感率均在30％以下.抗菌制剂外排泵基因oqxa、qaceΔl-sul1和mdfa的检出率很高,分别为100％、100％和65％,smrkpn、qace和teha检出率很低,分别为0％、0％和15％.结论 从烧伤科分离的多重耐药肺炎克雷伯菌中检出oqxa基因,且携带率非常高.
abstract：
objective to investigate the prevalence of multidrug resistant genes in klebsiella pncumoniae.methods twenty strains of multidrug resistant klebsiella pneumoniae were isolated from burn patients.susceptibility of these strains to 14 antibiotics was detected by kb method.pcr was used to detect oqxa,smrkpn,qace,teha,mdfa and qaceΔl-sul1 genes.results the antibiotic sensitivity rates of 20 multidrug resistant klebsiella pneumoniae isolates to antibiotics tested were ＜ 30％ except that to imipenam.the positive rates of efflux pump genes mdfa,qaceΔl-sull and oqxa were 65％,100％ and 100％,respectively; while those ofsmrkpn,qace and teha were 0％,0％ and 15％.conclusion oqxa gene has been detected in multidrug resistant klebsiella pneumoniae from burn patients with high positive rate.
