[目的]研究家蚕微孢子虫对果蝇的侵染性,为系统研究家蚕微孢子虫侵染机制提供新视野,也为探索微孢子虫的生物防治效果提供参考。[方法]用家蚕微孢子添食野生型和突变型果蝇,并用Calcofluor White M2R荧光染色法进行果蝇体液内的家蚕微孢子虫形态观察。[结果]结果表明,家蚕微孢子虫在转宿主情况下能够侵染果蝇,且被感染的突变型果蝇体内家蚕微孢子虫的数量多于野生型果蝇。[结论]家蚕微孢子虫能侵染果蝇,该研究结果为研究家蚕微孢子虫侵染其他宿主提供了初步参考,为进一步了解和认识家蚕微孢子虫侵染机制奠定了基础。
[ Objective ] The aim of this study was to investigate the infectivity of Nosema bombycis to drosophila, which offered a new vision for systematical studies on the infection mechanism of Nosema bombycis, and also provided reference for the bio-control effect of Nosema bombycis. [ Method ] Nosema bombycis was used to feed wild type and mutant drosophila, and the morphological observation of Nosema bombycis in drosophila body fluid was also analyzed by calcofluor white M2R fluorescent staining. [ Result] Nosema bombycis could infect drosophila, and the number of Nosema bombycis in the infected mutant drosophila was higher than that in wild type drosophila. [ Conclusion ] Nosema bombycis can infect drosophila, which provides primary reference for studies on the infectivity of Nosema bombycis to other hosts and also lays a foundation for further study on the infection mechanism of Nosema bombycis.
