At present,the progress of the study and application of forest plant disease was impeded by the lack of the internet resources of research achievements and control methods of forest plants disease.So a forest plant disease information manage system based on internet were needed.Chinese forest plant disease information website was established on the basis of HTML,ASP,Javascript and Microsoft Access 2000.The sources of the database were Chinese forest plant disease occurred severely in recent decades and the forest plant disease specimen of Chinese Academy of Forestry.The website was divided into six basic function modules,including the home page,the research group information,the guestbook,the forest plant disease information management system,the forest plant disease specimen management system and the forest plant disease diagnose system.The forest plant disease information could be easily queried and browsed through supported fields with these function modules.Meanwhile,the discussions about forest plant disease could be done with the guestbook or BBS.The information and achievements of the research group were also showed through the website.
【目的】克隆84K杨水杨酸结合蛋白2(Salicylic acid-binding protein 2,SABP2)基因并预测其功能。【方法】以生长至5片小叶的84K杨组培苗为材料,提取其叶和茎的总RNA。根据毛果杨SABP2基因(GenBank序列号:XM_002310718.2)的完整CDs序列,设计84K杨SABP2基因的引物,采用RT-PCR技术扩增84K杨SABP2基因全长,然后连接到pGM-T克隆载体,转化大肠杆菌TOP10感受态细胞,对84K杨SABP2基因进行克隆,获得该基因的全长序列。通过各种在线软件对84K杨SABP2基因及其编码的蛋白质进行生物信息学分析。【结果】84K杨基因的cDNA序列全长822bp,开放阅读框789bp,编码263个氨基酸。生物信息学分析表明,84K杨SABP2与毛白杨SABP2(GenBank序列号:JQ086570.1)的同源性最高,达98%;84K杨SABP2基因位于微体中;SABP2蛋白有11个蛋白质结合位点,属于α/β折叠水解酶家庭成员中的酯酶,为亲水性蛋白。【结论】成功克隆了84K杨的SABP2基因,其功能与前人对草本植物中SABP2部分功能的研究结果一致。
