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供职机构:红河学院生命科学与技术学院更多>>
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Preliminary Identification of Loquat Leaf Mould Pathogenic Fungus in Mengzi City of Yunnan Province
2013年
[Objective] The study aimed to investigate the Ioquat leaf mould disease in Mengzi City of Yunnan Province and lay the foundation for determination of effective prevention and control methods.[Method] Loquat leaf mould pathogenic fungus was isolated by tissue separation method and inoculated with conidial suspension.The pathogenicity of Ioquat leaf mould pathogen was verified by Koch's postulate.Under a microscope,mycelial morphology and conidial fructification were observed,spore sizes were measured,and Ioquat leaf mould pathogen was identified according to the morphological characteristics.[Result] Velvet-like,olive green fungal colonies were generated on PDA medium.Conidiophores erect,apex curved,dark brown,smooth,with obvious spore marks and no diaphragm,(33.0-152.8) μm×(2.6-4.0)μm.Cladosporium was brown or pale olive with spore marks,monocelled or with one diaphragm,(7.1-19.0) μm × (1.9-5.9) μm.Conidia concatenate (2-4),oval or spherical,with no spore mark,light olive,monocelled,smooth,(2.1-9.4) μm × (1.2-2.6) μm.[Conclusion] Loquat leaf mold disease began to occur in the germination period of spring shoots and summer shoots and became serious in the germination period of autumn shoots.Sooty mold-like layer grew on both front and back surfaces and densely covered the whole leaves,thus seriously affecting the photosynthesis of plants.The pathogen was preliminarily identified as Cladosporium eriobotryae Pass.& Beltrani.
杨若鹏张祖芸谢开美郑肖兰鲁海菊
关键词:LOQUAT
云南蒙自枇杷叶霉病病原菌的初步鉴定
2013年
为了进一步研究云南蒙自的枇杷叶霉病病害,并为确定其有效的防治方法奠定基础,病原分离采用组织分离法,通过分生孢子悬浮液接种,用柯赫氏法则对其致病性进行验证。显微镜下观察菌丝形态及产孢结构并测量孢子大小,根据形态特征进行鉴定,在显微镜下发现分生孢子梗直立,顶端曲屈,深褐色,无隔膜,平滑,孢痕明显,(33.0~152.8)μm×(2.6~4.0)μm。枝孢褐色或淡橄榄色,有胞痕,单胞或有1个隔膜,(7.1~19.0)μm×(1.9~5.9)μm。分生孢子链生(2~4),椭圆形,偶球形,无孢痕,淡橄榄色,单胞,平滑,(2.1~9.4)μm×(1.2~2.6)μm。结果发现,叶霉病在春梢和夏梢萌发期开始发病,秋梢发病最重,叶片正面和背面都长出烟煤状霉层,密布全叶,严重影响植株的光合作用,其病原菌初步鉴定为枇杷枝孢。
杨若鹏张祖芸谢开美郑肖兰鲁海菊
关键词:枇杷叶霉病病原菌鉴定
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