The aim was to promote the cultivation and application of medicinal plants Glehnia littoralis. [Method] Glehnia littoralis was used as experi-mental materials in this study, and orthogonal design was performed to investigate the effects of light, fertilizer and irrigation on the growth of Glehnia littoralis. The biomass, leaf chlorophyl and malondialdehyde （MDA） content were determined to study its response to light, fertilizer and irrigation. [Result] The results showed that the optimum cultivation conditions of Glehnia littoralis were not completely consistent with wild living environments. Lightly shade, highly fertilization （21.38-32.08 g） and proper irrigation （500-750 ml） were suitable for artificial cultivated Glehnia littoralis. Under the condition, the biomass was enhanced, leaf chlorophyl and carotene con-tent were increased, and MDA content changed significantly. Under stress condi-tions, the biomass of Glehnia littoralis reduced, with the decrease of chlorophyl and carotenoid content. The content of chlorophyl a was more sensitive to the variation of light, fertilizer, irrigation and other environmental factors, and MDA content signifi-cantly increased by stress conditions. [Conclusion] This research can provide refer-ence for large-scale artificial cultivation of Glehnia littoralis.
