目的:利用网络药理学方法和实验验证分析脊痛消胶囊治疗腰椎间盘突出症(lumbar disc herniation,LDH)的作用机制。方法:从中药系统药理学(Traditional Chinese Medicine Systems Pharmacology,TCMSP)、中药分子机理的生物信息学分析工具(Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine,BATMAN-TCM)、中医药资料@Taiwan和Swiss数据库中检索并筛选脊痛消胶囊的有效成分及其作用靶点,并使用GeneCards、人类孟德尔遗传(Online Mendelian Inheritance in Man,OMIM)和DisGeNET数据库预测LDH的疾病靶点,再将活性成分作用靶点与疾病靶点进行映射,得到脊痛消胶囊治疗LDH潜在靶点,继续在STRING数据库中进行蛋白互作分析(protein-protein interaction,PPI),将结果导入Cytoscape软件获取PPI网络图和“药物-活性成分-潜在靶点”网络图。利用clusterProfiler包对潜在靶点进行基因本体(Gene Ontology,GO)功能、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析,用Autodock Vina和Discovery Studio软件对活性成分和关键靶点进行分子对接验证。最后以脊痛消胶囊为干预因素,在LDH模型大鼠进行实验验证。结果:网络药理学结果显示,共收集到脊痛消胶囊活性成分139种、靶点479个,获得LDH靶点590个,映射得到84个交集靶点,通过PPI网络筛选得到脊痛消胶囊治疗LDH的关键治疗靶点10个:信号转导和转录激活因子3(signal transducer and activator of transcription 3,STAT3)、转录因子JUN(transcription factor Jun-1,JUN)、白细胞介素(interleukin,IL)-6、IL-10、丝裂原活化蛋白激酶1(mitogen-activated protein kinase 1,MAPK1)、FOS蛋白(protein c-fos,FOS)、连环素(catenin beta-1,CTNNB1)、丝裂原活化蛋白激酶14(MAPK14)、蛋白激酶B(protein kinase B,PKB/AKT1)和肿瘤坏死因子(tumor necrosis factor,TNF),GO功能富集分析显示交集靶点涉及2163种生物学过程、36种细胞组分和102种分�
在膝骨关节炎的发生发展过程中,素体阳虚和外邪侵袭是导致本病的重要原因,过往的中医学研究中,大多用活血化瘀,祛风散寒的方法治疗,基于温阳法治疗寒湿痹阻型膝骨关节炎的研究较少。我国东北地区气候寒冷,寒湿痹阻型膝骨关节炎占多数,高曦主任医师认为素体阳虚,寒邪侵袭为膝骨关节炎的主要原因,今通过温阳补血,散寒通滞的方法探讨温阳法在膝骨关节炎治疗中的应用,以丰富中医药治疗膝骨关节炎的治疗方法,为未来临床和科研提供新思路。In the development of osteoarthritis of the knee, physical Yang deficiency and external attack are important causes of this disease, past Chinese medicine research, most of the blood circulation and blood stasis, wind dispersal method of treatment, based on the warming Yang method of treatment of cold-damp paralytic osteoarthritis of the knee is less research. In the cold climate of northeast China, cold-damp paralytic osteoarthritis of the knee accounts for most of the cases. Dr. Gao Xi, Chief Physician of the Department of Medicine, believes that yang deficiency of the vegetative body and the invasion of cold are the main causes of osteoarthritis of the knee, and discusses the application of the method of warming yang in osteoarthritis of the knee by warming yang to replenish the blood and dispersing the cold and stagnation to enrich the therapeutic methods of traditional Chinese medicine in the treatment of osteoarthritis of the knee, and to provide a new way of thinking for the future clinical and scientific research.
