分析了激素对两个基因型小麦幼穗组织培养效果的影响,结果表明,愈伤组织诱导率在每个组合中均可达100%,诱导培养基中加入2,4-D对植株再生和绿点分化频率的效果要显著优于P icloram,在所采用的有效2,4-D浓度范围内,随着浓度的升高,绿苗的再生频率呈现上升的趋势,2,4-D的量为4.5 m g/L时,植株的再生频率平均可以达到45%左右.
Studies were carried out to establish an efficient regeneration system of three bread wheat cultivars. Results showed induction medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) had a higher plantlet regeneration frequency than Piclorm, with an average frequency of 54% in all treatments. Optimal condition for different genotypic rice was as following: induction medium (MSS 3AA/2) with 0.5 mg L-1 2,4-D, regeneration medium (R) with 0.01 mg L-1 2,4-D and 3 mg L-1 KT. The average regeneration frequency reached 83.3% under the condition. Correlation analysis showed that root differentiation, in different level, correlated with green spot regeneration, and with the number of regenerated plants per callus. No correlation was found between green spots regenerated and the numbers of plants regenerated per callus.
