A partial common carp genomic library was constructed.45 positive clones were isolated from screening about 2000 clones of the genomic library with a (CA) 15 probe labelled at the 5’ end with γ 32 P ATP.Sequencing of these clones was performed with automated sequencer,and 22 microsatellites were isolated.17 primers were designed based on unique sequences flanking each motif with the software Primer3.PCR on Cyprinus carpio haematopterus was carried out with these primers,and all gave expected bands.Annealing temperature of these primers was between 50℃ and 53℃.
萍乡红鲫又名萍乡肉红鲫,英文名Pingxiang red transparent crucian carp,拉丁学名Carassius auratus var pingxiangnensis,它是继江西“三红”(兴国红鲤、婺源荷包红鲤、万安玻璃红鲤)之后江西第四个全红鲤、鲫品种,也是继彭泽鲫之后江西自主选育的第二个良种鲫。