To study the morphology and physiology of blood cells from five species of heterotherm vertebrates（carp Cyprinus carpio,crucian Carassius auratus,catfish Silurus asotus,toad Bufo bufo gar-garizans and Chinese soft-shelled turtle Pelodiscus sinensis）,three kinds of living cells（thrombocytes,small lymphocytes,erythrocytes）were obtained from the experimental animals.Plasma samples were partly anticoagulated with trisodium citrate,potassium oxalate and magnesium sulphate（MgSO4）at 23℃ in vitro,and observed in the light microscope and scanning electron microscope.Results showed that:1)small lymphocytes were typically spherical and normally dispersed in oxalate-plasma from all of the experiment animals and in citrated-plasma from carp and crucian.However,they were aggregated in MgSO4-plasma from all of the experiment animals and in citrated-plasma from catfish,toad and Chinese soft-shelled turtle.2)thrombocytes were dispersive and spindle or elliptical in shape in oxalate-plasma from the five species of experimental animals;in citrated-plasma from carp,crucian and catfish;in MgSO4-plasma from carp,crucian,catfish and Chinese soft-shelled turtle.However,they were aggregated in citrated-plasma from toad and Chinese soft-shelled turtle,and in the MgSO4-plasma from toad.3)erythrocytes were typically normal,and not haemolytic in citrated-plasma and in MgSO4-plasma from five species of experimental animals,but they were completely haemolytic in oxalate-plasma from experimental animals except for toads.Rouleaux formation of erythrocytes,such as occurs in mammalian plasma,was not observed in the experimental animals.We conclude that:1)the same of small lymphocytes or the thrombocytes in heterotherm vertebrate plasma possess the ability to aggregate together in vitro,the process of the aggregation may relate to plasma Ca 2+ and Mg 2+ concentration;2)the process of erythrocyte sedimentation caused by their specific gravity in plasma of these experimental animals,is different from that caused by rouleaux formation of
[Objective] The research aimed to determine the existence of free phospholipids in the plasma of three species of freshwater fish and lay the foundation for discussing the physiological mechanism of coagulation phenomena in three species of freshwater fish.[Method] The activity of heated phospholipids was detected by the availability test of heated rabbit cephalin.Small amount of the platelet-poor plasma of the rabbit Oryctolagus cuniculus,the cell-free plasma of the carp Cyprinus carpio,the crucian Carassi...
