A long procedure is needed to quarantine pinewood nematodes because only the dispersal third-stage larvae could be isolated and it cannot be identified directly before being cultured to adult nematodes. To shorten the procedure, the effects of different temperatures and culture durations on the development of B. xylophilus larvae were studies with three fungus species as the culture media. The result showed that temperature had differential influence on development of the larvae in the three fungus media, and the influence was not significant. There was significantly different effect of the cold-stored fungi media on the larvae development. Fusarium sp. was the best, Pestalotia sp. was the second, Botrytis cinerea was the worst in promoting larvae development. Cold-stored B. cinerea was not suitable for the rapid quarantine of the nematode. When the L_Ⅲ larvae of B.xylophilus were abundant the quarantine could be accomplished in 48 h by culturing the L_Ⅲ larvae with cold-stored Fusarium sp. or Pestalotia sp. at 30
