In this paper,the noncovalent interactions between α1-acid glycoprotein（AAG） and the drugs,such as ketoprofen（KP） and anisodine hydrobromide（AN） were studied by using electrospray ion trap mass spectrometry method.The results show that both of the drugs had a strong coalescent interaction with AAG,the（dissociation） constants（Kd） and the apparent stoichiometric ratio of noncovalent complexes AN-AAG were（1.75×10-3 mol/L） and 1∶7,and those for KP-AAG complex were 4.95×10-4 mol/L and 1∶4,respectively.According to the thermodynamic parameters of the noncoralent complexes,the main sorts of binding force were static-electricity gravitation for KP-AAG and Van der Waals force for AN-AAG,respectively. The experiment proves that ESI-MS technology as a new method for studying the drug-protein noncoralent binding can offer some advantages in sensitivity,speed,accuracy and directness particularly in the determination of the stoichiormetry of the complex compared to other well-established methods such as circular dichroism and fluorescence spectroscopy,etc.
目的研制麻疹-流行性腮腺炎(流腮)-流行性乙型脑炎(乙脑)联合减毒活疫苗(Measles,Mumps and Japanese Encephalitis Combined Attenuated Live Vaccine;M-M-JEV)。方法选用麻疹病毒沪191疫苗株、流腮病毒Wm84疫苗株和乙脑病毒SA14-14-2疫苗株,制备单价麻疹减毒活疫苗(Measles Attenuated Live Vaccine,MV)、流腮减毒活疫苗(Mumps Attenuated Live Vaccine,MuV)和乙脑减毒活疫苗(Japanese Encephalitis Attenuated Live Vaccine,JEV)原液,进行干扰试验、配比试验、保护剂筛选试验、冻干曲线的选择。结果确定了M-M-JEV的制备工艺,进行了M-MJEV的中试。中试的M-M-JEV经全面检定,各项指标均符合2010年版《中华人民共和国药典》(《中国药典》)三部单价MV、MuV和JEV的质量标准。动物试验未见病毒间交叉干扰,未见加重反应,未影响小鼠的免疫应答,表现出了良好的安全性和免疫原性。结论成功制备了M-M-JEV。
目的考察麻疹-流行性乙型脑炎（乙脑）联合减毒活疫苗（Measles and Japanese Encephalitis Combined Attenuated Live Vaccine,M-JEV）的稳定性。方法将M-JEV,37℃放置7d,检测病毒滴度;2-8℃放置0-24个月,检测外观、水分、病毒滴度、安全试验、异常毒性,0月和18个月时进行全检;（-30±2）℃放置0-30个月,检测外观、水分、病毒滴度、安全试验、异常毒性,0月、18个月、30个月时进行全检。结果所有检测结果,均符合2010年版《中华人民共和国药典》三部麻疹减毒活疫苗和乙脑减毒活疫苗的质量标准。结论 M-JEV有较好的稳定性。
目的考察麻疹-流行性腮腺炎（流腮）-流行性乙型脑炎（乙脑）联合减毒活疫苗（Measles,Mumps and Japanese Encephalitis Combined Attenuated Live Vaccine;M-M-JEV）的稳定性。方法将M-M-JEV,37℃放置7d,检测病毒滴度;2-8℃放置0-25个月,检测外观、水分、病毒滴度、安全试验、异常毒性,0月和18个月时进行全检;（-30±2）℃放置0-30个月,检测外观、水分、病毒滴度、安全试验、异常毒性,0月、18个月、30个月时进行全检。结果所有检测结果,均符合2010年版《中华人民共和国药典》三部麻疹减毒活疫苗、流腮减毒活疫苗和乙脑减毒活疫苗的质量标准。结论 M-M-JEV有较好的稳定性。
