To study the inhibition effect of oxLDL on the uptake and clearance of intra ce llular 3H cholesterol in v SMC from the human apoA1 transgenic m ice (C57BL/6) and the changes in human apoA1 mRNA expression in v SMC from hum an apoA1 transgenic mice after oxLDL stimulation Methods v SMC originally isolated from human apoA1 transgenic mice connected with a rec ombined mouse metallothionein Ⅰ (MT Ⅰ) promoter was used, and the effect of oxLDL on the uptake and clearance of intracellular 3H cholesterol was s tudied in v SMC of the transgenic and control mice respectively, the study of h apoAⅠ mRNA expression from v SMC of the transgenic mice were done by RT PCR and Northern blot Results oxLDL (30?μg/ml) strongly promoted the SMC proliferation No difference was f ound in 3H cholesterol uptake between nSMC and trSMC, and the uptak e rates of both kinds of SMC rose 100% after oxLDL stimulation The efflux rate s of 3H cholesterol in trSMC were much higher than those of nSMC (40% -50%) Afte r ox LDL stimulation, the clearance rates fell by 28% and 10%, respectively, for nSMC and trSMC The result of RT PCR and Northern blot showed that h apoA1 gene e xpression was markedly increased by the stimulation of oxLDL Conclusion Expression of the h apoA1 gene in C57BL/6 mice enables them to reduce the accum ulation of cholesterol in v SMC The trSMC can alleviate the harmful effect of oxLDL due to the increase of h apoA1 expression
