By using PCR and DNA recombination,two fusion genes of humanized mouse anti human fibrin scFv and low molecular weight single chain urokinase (Scu PA 32K)was constructed.The difference of these two fusion genes lay in the linker between two moieties,one was (Ala) 3 and another was (Gly 4Ser) 3.These two fusion genes were both overexpressed in E.coli with the expression level at 30%.Both expression products showed the activity of binding antigen D Dimer and activating plasminogen after the denaturation and renaturation,but under general refolding conditions,the one with linker (Gly 4Ser) 3 showed better effect in the renaturation of fusion protein.
