[Objective] The paper was to study the karyotype of Chi-lin fish （Varicorhi- nus macrolepis） from Taishan mountain. [Method] The chromosome number and karyotype of an endemic Chi-lin fish were studied using the head kidney cells. [Re- sult] The diploid chromosome number （2n） of Chi-lin fish was 50, and the karyotype of V. macrolepis was 2n=50,16m＋14sm＋20t, NF =80. No sex-chromosome was found in Chi-lin fish. [Conclusion] A detailed karyotype of this endemic cyprinid fish of Taishan Mountain was established for the first time in this study.
