Traditional Chinese Medicine (TCM) based on natural products is one important part of the Chinese civilization. Owing to the complexity of the composition, the study on medical effective components and curative effects are very difficult. Fuzi (Radix Aconiti Lateralis Praeparata) has been widely used for cardiotonic and analgesics in China,however, the component-aconitine in which is very toxic and may cause some side-effect.This paper reported the application of electrospray iohization mass spectrometric(ESIMS)technique on study of the compound prescriptions containing fuzi, Renshensini concoction and Baweidihuang concoction, to explore the mechanism of synergy between fuzi and other herbs. From chemical point of view, the detoxification mechanism can be attributed to the hydrolysis of the solubility of toxic diester-alkaloids from concoctions of fuzi. Radix Glycyrrhizae Praeparata, Rhizoma Zingiberis, Radix Ginsengp promote the hydrolysis to produce the less toxic monoester-alkaloids and Fructus Corni reduce the solubility of hypa-conitine from fuzi. However pinellia fritillary and bletilla were forbidden to use with fuzi,because they suppress the hydrolysis of fuzi in the solution. This work applied electrospray ionization mass spectrometry, tandem mass spectrometry (MSn) , high performance liquid chromatography (HPLC) techniques to study the reactions (including hydrolysis and ester-exchange reaction etc. ) occurred during the decoction and the concoction process via the gas phase ion chemical reactions and discuss the conditions to the reactions. On the other hand, the fragmentation mechanism of aconitine alkaloids in mass spectrometry was studied as well.
硒结合蛋白1(Selenium-binding protein 1,SBP1)与肿瘤相关,并可以与重金属镉直接结合。该研究构建了白鳍鲨SBP1重组质粒Myc-SBP1并转染人HL-7702细胞,利用CCK-8和CFDA SE检测Cd胁迫下的细胞增殖情况,利用流式细胞仪检测细胞内ROS的水平。结果显示,过表达白鳍鲨SBP1可拮抗Cd对HL-7702细胞的毒害作用。在100μmol/L的CdCl2应激下,表达SBP1组存活率为76.4%,约为对照组存活率的2倍,实验组的细胞增殖指数和分裂能力也略高于对照组。一级结构分析表明,白鳍鲨SBP1具有两个CXXC区域,为抗氧化位点,具有2个H×D和3个HXXH保守区域,它们为推测的Cd结合位点,为在HL-7702细胞中表达白鳍鲨SBP1蛋白能拮抗Cd的结构基础。此外,ROS检测实验表明白鳍鲨SBP1蛋白能显著降低胞内ROS水平,提示硒结合蛋白除通过直接与Cd结合降低毒性外,降低重金属引起的氧化胁迫也可能发挥一定解毒作用。
