In order to look for Trichderma strains which have inhibiting effects on Cytospora chrysosperma, total 29 domestic and foreign Trichderma strains were screened by confrontation culture and growth rate method. The physiological ecology characteristics and taxonomic statuses of the screened highly efficient antifungal strains were studied. The results showed that the n-butanol extract from T-33 fer- mentation liquid showed the highest inhibition rate （94.2%） against mycelial growth, and its inhibition rate against spore germination was up to 100%. For the growth of T-33 strain, the optimum carbon source was glucose with optimum content of 3% （g/L）; the optimum nitrogen source was soy four with optimum content of 0.02% （g/L）; the optimum initial pH of culture medium was 6; the optimum culture temper- ature was 25 ℃. The 18S rDNA sequencing results showed that the full length of ITS sequence was 583 bp, and the T-33 strain was verified as Trichoderma viride.
为了寻找对杨树烂皮病病原菌具有高效抑制作用的木霉菌株,采用对峙培养法和生长速率法对国内外29个木霉菌株进行了筛选,并对筛选出的高效菌株的生理生态特性和分类地位进行了研究。结果表明:菌株T-33发酵液正丁醇提取物对病原菌菌丝生长的抑制率最高,达到94.2%,对孢子萌发抑制率达到100%。菌株T-33生长最适碳源为葡萄糖,最佳用量为3%(g/L);最适氮源为黄豆粉,最佳用量为0.02%(g/L);培养基最适初始p H 6;最适培养温度25℃。通过18S r DNA测序分析,ITS序列长度是583 bp,菌株T-33鉴定为Trichoderma viride。
