运用一对特异性引物扩增并测定了南海硬骨鱼类40个物种89个样本的线粒体DNA的细胞色素c氧化酶Ⅰ(COⅠ)约660bp的部分序列,即COⅠ条码序列。综合比较了《中国鱼类系统检索》、《海洋鱼类志》、FishBase鱼类形态学分类库、ITIS综合分类学信息系统和生物条码系统(The Barcode of Life Data System,BOLD)的相应形态学与DNA序列资料,在揭示完善我国鱼类检索系统必要性基础上,探讨了COⅠ条码序列在硬骨鱼类辅助物种鉴别和适用性,并对运用该条码序列库完善我国鱼类检索系统的可能途径进行了初步摸索。结果表明,COⅠ条码序列获取便捷,广泛适用硬骨鱼类物种鉴别,并可用于低级分类阶元的系统进化分析。本研究结果可为分子生物学辅助分类、市场监督、资源有序利用和保护提供参考。
This paper deals with two chemical disinfectants on the growth and metabolism of Nannochloris oculata .The results show that at both the concentrations of availability Cl≥2.5mg/L and availability I≥0.2 mg/L and with the concentration of the chemical disinfectant, the growth rate, the Chlorophylla content, photosynthesis and respiration were reduced markedly. The higher the concentration is, the greater the toxicity will become. At the concentrations of availability Cl≥4.5 mg/L, Nannochloris oculata degrades markedly. Nannochloris oculata appears "toxicity excitement result"in the low levels Cl.
