[Objective] The paper was to study the nitrogen application amount and nitrogen application model for high grade hybrid rapeseed (Brassica napus L.) to get high yield. [Method] With "Youyan 599" and "Sanbei 98" as materials, using quadratic regression orthogonal gyration combination design, the impact of nitrogen application amount during various periods on rapeseed yield was studied. [Result] The combinations of factors to obtain the highest yield index (2 898.211 kg / hm 2 ) of "Youyan 599" were as follows: living rape fertilizer 89.27 kg / hm 2 , opening fertilizer 120 kg / hm 2 , 12 th lunar month fertilizer 101.12 kg / hm 2 , total nitrogen application amount 310.39 kg / hm 2 . The proportions of living rape fertilizer, opening fertilizer and 12th lunar month fertilizer were 28.76%, 38.66% and 32.58%, respectively. The combinations of factors to obtain the highest yield index (2 870.14 kg/hm 2 ) of "Sanbei 98" were as follows: living rape fertilizer 120 kg / hm 2 , opening fertilizer 120 kg / hm 2 , 12 th lunar month fertilizer 37.55kg / hm 2 , total nitrogen application amount 277.55 kg / hm 2 . The proportions of living rape fertilizer, opening fertilizer and 12 th lunar month fertilizer were 43.24%, 43.24% and 13.53%, respectively. The combinations of factors to obtain the highest yield index of two combined varieties (2 813.82 kg/hm 2 )were as follows: living rape fertilizer 120 kg/hm 2 , opening fertilizer 120 kg/hm 2 , 12 th lunar month fertilizer 76.23 kg/hm 2 , total nitrogen application amount 316.23 kg/hm 2 . The proportions of living rape fertilizer, opening fertilizer and 12 th lunar month fertilizer were 37.95% , 37.95% and 24.11% , respectively. [Conclusion] The paper provided theoretical basis for high yield cultivation of high grade hybridized rapeseed.