Electrophoresis and staining of proteins from the single pollen grains of Hibiscus rosa sinensis have been developed by using general ultrathin polyacrylamide gel combined with highly sensitive silver staining technique.The result revealed that the pollen abortion could occur in different stages of pollen development.The protein patterns varied greatly in different stages of pollen development, even in the different pollen grains in the same anther at the same development stage.Some bands exhibited a disjunction by classical Mendelian ratio 1∶1, suggesting that the gene loci were heterogeneous and the proteins were related to the expression of the genes at the early stage of pollen development.
离体条件下风信子(Hyacinthus oricntalis L.) 再生雄蕊的小孢子发生和雄配子体发育的细胞学观察表明:从四分体到二细胞花粉,二者的细胞学特点基本一致。发育到成熟花粉时,再生雄蕊的成熟花粉是三细胞的,自然情况下是二细胞的。表明了两者在生殖细胞分裂完成的时间和完成时在细胞中所处的位置等方面都存在明显的差异。对这种差异产生的原因及是否会对精细胞产生影响等方面进行了讨论。
