The genomic library of a cold-resistant fresh water teleost, Leuciscus waleckii,was constructed by using phage EMBL3 as vector. Three positive liybridized recombinant phage plaques were screened out by AFP gene probe of a marine teleost, North American winter flounder(Pseudopleur-onectes americanus).Three positive hybridized bands of 2.7 Kb/Sac I, 8 Kb/BamHI and 20 Kb/Hind III DNA segments obtained from these positive hybridization recombinant phage DNA were identified when a AFP gene and a 0.4 Kb cDNA of AFP gene of a marine teleost,the winter flounder, were used respectively as the probes for Southern analysis.These preliminary results show that a homologous sequence of AFP gene of winter flounder seems to be present in the genome of Leuciscus waleckii.
