The diagnostic methods of extracelluar oxidase mat were used to select lignin-degrading enzymes producers among nineteen Ganoderma lucidum fungal strains.The results showed that the producing laccase activity of G.sp.var.Tianzhi was the highest,followed by that of G.lucidum 10 and G.lucidum 8.The producing lignin peroxidase activity of G.sp.var Tianzhi was the highes,and the producing Manganese peroxidase activity of G.sp.var yuanzhi 6 and G.lucidum G 8 were the highest,followed by that of the G.sp.var.Tianzhi and G.lucidum 10.It was found that G.sp.var Tianzhi,G.sp.var.Yuanzhi 6G.lucidum 8 and G.lucidum 10 showed the highest lignin-degrading enzymes activity.
Effect of cadmium(Cd) on the uptake of mineral nutrient in Salit matsudana was investigated in hydroponic culture with different Cd treatments(0,5,25,50 μmol·L-1) for 14 d.Cd treatment did not significantly affect K uptake in roots,in leaves,K content was increased by 5 μmol·L-1 Cd,however,no significant change was observed when treated with 25-50 μmol·L-1 Cd.In contrast,P uptake in roots was inhibited at all Cd levels,P content in leaves was decreased by 25-50 μmol·L-1 Cd.Ammonium content was elevated in roots but decreased in leaves under all Cd treatments.Nitrate content of roots and leaves showed a slight increase compared with the control.In roots,low Cd(5 μmol·L-1) enhanced the increase in NR activity,but didn’t markedly change at 25-50 μmol·L-1 Cd,NR activity of leaves was reduced in all Cd levels compared to the control.Concentrations of Zn and Mn in roots and leaves decreased with the increase of Cd in the medium,Fe concentration in roots was reduced by Cd,but translocation of Fe from roots to leaves increased in high level of Cd,Cu content was little influenced by Cd.These results suggested that Cd toxicity led to disruption of mineral nutrient uptake in S.matsudana.
