Adding sodium chloride into a medium containing 140g·L - 1 glucose could promote the glycerol accumulation efficiently, but the glycero l productivity would decrease when sodium chloride concentration was above 40g· L -1.Experimental results in shake flask showed that sodium chloride conce ntration of 40g·L -1 was favorable.Moreover, the feasibility of multi-st age cascade continuous fermentation was tested and the results indicated that mu lti-stage continuous system was superior to single-stage continuous fermentati on.Compared with batch fermentation,the glycerol concentration and the glycerol yield in multi-stage cascade continuous system decreased by only 9.83 % and 5 .71 % respectively, but the glycerol productivity increased by 18.54% even the preparing time between two batch fermentation was not considered..
