[Objective] This experiment aimed to evaluate the effects of calcium and Jasmonic acid（JA） on the expression of CBF in spinach. [Methods] The seedlings of spinach were treated with low temperature （4 ℃）, JA or A23187, then used for detecting the expression of CBF by northern blotting. [Results] The results showed that the CBF expression was regulated by low temperature and JA positively. [Conclusions] Low temperature may increase the JA content of the cell firstly, then JA induced the increase of cytosolic calcium concentration （[Ca2＋]cyt）, and the JA induced Ca2＋ transmitted the low temperature signal through CaM or CaM related proteins, regulating the CBF expression.
[Objective] This study was to investigate the role of IP3 sensitive calcium channel in the JA-induced calcium mobilization pathway.[Method] Arabidopsis thaliana leaves were labeled by Fluorescent probe Fluo-3/AM under low temperature at 4 ℃ to measure the fluorescent intensity of intracellular Ca2＋ which was pretreated with heparin on jasmonic acid（JA）-induced.[Results] When A.thaliana leaf cells were pretreated with 10,50 or 100 ng/ml heparin,intercellular free Ca2＋ fluorescence intensity was reduced in comparison with negative control.Once the heparin-pretreated A.thaliana leaf cells were stimulated with 100 μmol/L JA,intercellular Ca2＋ fluorescence intensity increased gradually and tended to be stable at a degree equivalent with that in negative control.[Conclusion] The experiment showed that the pretreatment with heparin could inhibit the increase of the intracellular Ca2＋ concentration significantly which JA-induced in leaves of Arabidopsis thaliana.
