本研究探讨多壁碳纳米管为载体组装金粒子及乙脑疫苗抗体,制备无电子媒介体乙脑疫苗免疫传感器的可行性及其基本性能。采用恒电位法在碳纳米管表面沉积金微粒,再利用物理吸附在金微粒表面固定乙脑疫苗抗体,并通过辣根过氧化氢酶封闭传感器上的非特异性活性位点,利用其对H_2O_2的反应增强电流信号,制得乙脑免疫传感器。循环伏安法被用来对制备各阶段电极进行表征,多种电化学方法详细研究了免疫传感器性能。实验结果表明该免疫传感器在5.6×10^(-8)～2.2×10^(-6) lg pfu/mL的范围内保持良好的线性关系,具有较高的灵敏度(72.4μA/lg pfu/mL/cm^2),较好的稳定性和选择性,具有潜在应用价值。
The electrochemical behavior of riboflavin on the multiwalled carbon nanotube modified electrode was investigated by cyclic votammetry.The results indicated that and the production by reduction reaction can be strongly adsorbed on the modified electrode.One pair of prewaves the multiwalled carbon nanotube modified electaode had an obvious electaocatalytic effect on riboflavinand one pair of two-electron redox waves were exhibited at low concentration and low scan rates.The peak current was proportional to the concentration of riboflavin over the concentration range from 1.0×10-5 to 1.0×10-4mol/L with correlation coefficient of 0.99757.
首次以高分子量的聚(L-乳酸-co-乙醇酸)(PLLGA)和D-聚乳酸(PDLA)[m(PLLGA)∶m(PDLA)=3∶1,c 50 m L·g-1]为原料,氯仿为溶剂,等体积的甲醇为沉淀剂,于50℃蒸发4 h形成了PLLGA和PDLA的立构复合物(sc-PLA),其结构和性能经XRD,DSC和TGA表征。结果表明:sc-PLA的结晶度达96.2%,热失重5%温度为342℃(PLLGA为304℃)。
