Objective:To establish an ovarian carcinoma multidrug resistance (MDR) cell line in order to explore the multidrug resistant mechanism in ovarian carcinoma and to understand the development of MDR and possible ways to overcome this phenomenon.Methods:SKOV 3/ADM,a MDR cell line,was established by SKOV 3 parent cells exposed to progressively and intermittently increasing higher concentration of adriamycin (ADM) and had been identified.Results:Besides the drug resistance to adriamycin, the SKOV 3/ADM cells exhibited crossresistant to many other chemotherapeutic agents ,such as VM-26,VP-16 and others.The emergence of drug-resistant SKOV 3/ADM cells was mainly caused by the decreased intracellular drug concentration as a result of overexpression of P-glycoprotein encoded by MDR 1 gene.Conclusion:This cell line showed the typical multidrug resistance phenotypes and might serve as an ideal model for studying the mechanism of MDR.
