Objective To observe the migration and differentiation of the neural precursor cells （NPCs） that derived from murine embryonic stem cells （ESCs） when they were transplanted into amyloid β （Aβ）-treated rat hippocampus. Methods MESPU35, a murine ESC cell line that express the enhanced green fluorescent protein （EGFP）, was induced differentiation into nestin-positive NPCs by modified serum-free methods. The Aβ plaques and the differentiation of the grafted cells were observed by immunofluorescent staining. Results Comparing 16 weeks with 4 weeks post-transplantation, the migration distance increased about 5 times; the rate of migratory NPCs differentiating into glial fibrillary acidic protein （GFAP）-positive cells kept rising from （30.41 ± 1.45）% to （49.25± 1.23）%, and the rate of NPCs differentiating into neurofilament 200 （NF200） positive cells increased from （16.68±0.95）% to （27.94± 1.21）%. Meanwhile, the GFAP-positive cells targeting to the ipsilateral side of Aβ plaques increased from 60.2% to 81.3 %, while the NF200-positive cells increased from 61.3% to 84.1%. The migration distance had significant positive linear correlations to the neuronal differentiation rate （r = 0.991） and to the astrocytic differentiation rate （r = 0.953）. Conclusion Engrafted NPCs migrate targetedly to the Aβ injection site and differentiate into neurons and astrocytes.
目的:探讨丹皮酚和芦丁的联合应用对人鼻咽癌CNE-2细胞增殖的影响。方法:以不同浓度的丹皮酚、芦丁和两药的联合应用处理人鼻咽癌CNE-2细胞,采用MTT测量药物对体外培养的人鼻咽癌CNE-2细胞的增殖抑制作用,应用两药相互作用数(coefficient of drug interaction,CDI)进行联合用药分析。结果丹皮酚(12.5～200mg/L)和芦丁(5～80mg/L)单独应用均对人鼻咽癌CNE-2细胞有增殖抑制作用,呈剂量效应关系。丹皮酚和芦丁联合应用时,低浓度则有增殖抑制作用,但其作用比单种药物弱;高浓度对人鼻咽癌CNE-2细胞的增值有促进作用。总的来讲,两药作用性质为拮抗。结论:丹皮酚和芦丁联合抗癌研究显示,两药抗癌作用性质为拮抗,呈剂量效应关系。
