Sinopodophylli Fructus is the commonly used traditional Tibetan medicinal herb. In the present study, we established a reversed-phase high performance liquid chromatography method to simultaneously determine three lignans and five flavonoid constituents, namely podophyllotoxin, desoxypodophyUotoxin, 4＇-demethyldesoxypodophyllotoxin, 8-prenylkaemferol, quercetin, kaempferol, 8,2＇-diprenylquercetin 3-methylether and 8-prenylquercetin, in Sinopodophylli Fructus. The chromatographic separation was achieved on a C_18 analytical column with a gradient mobile phase consisting of acetonitrile and 0.05% phosphoric acid at a flow rate of 1.0 mL/min. UV detection was set at 290 nm and 370 rim, and the column oven was set at 35℃. This method provided a good reproducibility, and its overall intra- and inter-day precision was less than 3% and 4%, respectively. The recovery of the method was 98.29%-101.60%, and a good linearity （R2≥0.9992） was obtained for all the analytes over a relatively wide range of concentration. A total of 17 samples ofS. hexandrum （12 fruits, 5 roots and rhizomes） were collected from different areas and then successfully quantified. The results indicated that the contents of eight compounds significantly varied （the sum content ranged from 16.90 to 55.68 mg/g）, and prenylated fiavonoids could be used as marker constituents in the identification and quality control of Sinopodophylli Fructus.
