Singlet oxygen （^1O2） is widely considered to play a major role in photodynamic therapy （PDT）, and thus an increasing attention has been focused on the direct detection of ^1O2 near-infrared luminescence around 1270 nm for PDT dosimetry. A new sensitive detection system is developed to directly measure the temporal and spectral resolved ^1O2 luminescence spectra. The triplet state and ^1O2 lifetimes of Rose Bengal as a model photosensitizer in different solvents are determined, and the obtained results agree well with the published data. Our detection system has the potential application in ^1O2 luminescence-based PDT dosimetry.
Singlet oxygen(^(1)O_(2))is a highly reactive oxygen species involved in numerous chemical and photochemical reactions in diferent biological systems and in particular,in photodynamic therapy(PDT).However,the quantifcation of^(1)O_(2) generation during in vitro and in vivo pho-tosensitization is still technically challenging.To address this problem,indirect and direct methods for^(1)O_(2)detection have been intensively studied.This review presents the available methods currently in use or under development for detecting and quantifying^(1)O_(2) generation during photosensitization.The advantages and limitations of each method will be presented.Moreover,the future trends in developing PDT-^(1)O_(2) dosimetry will be briefly discussed.
