Based on the pathophysiology of the brain,advance in angiogenesis induced by stroke,and evidences of Chinese-medicine-mediated angiogenesis,the possibility to study the stroke-treating mechanism of Chinese medicine in angiogenesis was discussed.And regarding our previous work on angiogenesis modulated by qi-tonifying and stasis-eliminating therapy following intracerebral hemorrhage,we proposed some questions,which should be taken into account in the further work.
Neurogenesis and angiogenesis can improve the neurologic function after intracerebral hemorrhage(ICH).Leukemia inhibitory factor(LIF)plays an important role in neurogenesis and angiogenesis.In this study,a rat model of autologous blood-induced ICH was used to evaluate the effect of LIF on the neurogenesis and angiogenesis following ICH.After ICH,LIF-positive neurons and dilated vessels were detected in the peri-hematomal region.It was found that LIF levels increased significantly and peaked 14 days after ICH induction.Double immunofluorescence confirmed that LIF was expressed in neurons and endothelial cells.ICH also led to increases of doublecortin(DCX)-and von Willebrand factor(vWF)-positive cells as well as proliferation of cell nuclear antigen(PCNA)+/DCX+and PCNA+/vWF+nuclei.All these ICH-induced increases were significantly attenuated by exogenous LIF in fusion.These data suggested that LIF was a negative regulator of neurogenesis and angiogenesis after ICH.
Chuan-zhen LIUHua-jun ZHOUJian-hua ZHONGTao TANGHan-jin CUIJing-hua ZHOUQiang ZHANGZhi-gang MEI
