Au nanorods were prepared by a seeding growth approach and used in fabricating the nanorod-enhancing glucose biosensor.The high affinity of chitosan for Au nanorods associated with its amino groups resulted in the formation of a layer of Au nanorods on the surface of Au electrode.It served as an intermediator to retain high efficient and stable immobilization of the enzyme.The performance of biosensors was investigated by cyclic voltammetry (CV),in the presence of artificial redox mediator,ferrocenecarboxaldehyde.The biosensor had a fast response to glucose,and the response time was less than 10 s.The results indicated that the gold nanorods could enhance the current response to glucose.The detection limits of glucose can reach 10 mM,and the Michaelis-Menten constant K mapp is 13.62 mM.
LI YingyingWANG XiaoxiaDONG XiangHE ZhipengZHANG Haiqian
为了研究反应时间、温度、钙磷比以及反应体系的pH值对羟基磷灰石结构及形貌的影响,本文采用水热法在醋酸钙和磷酸的混合酸性溶液中制备羟基磷灰石。用扫描电镜(Scann ing e lectron m icroscopy,SEM)、X射线衍射(X-ray d iffraction,XRD)和红外光谱(FT-IR)法,对制备的羟基磷灰石进行了表征和分析。结果表明:生成的羟基磷灰石晶须的长度及长径比随反应时间的延长而增大。温度低于150℃时,得到羟基磷灰石晶须;温度高于180℃,则得到细小羟基磷灰石晶体的聚集产物。当反应物的pH=9时,得到的是缺钙型的羟基磷灰石;当反应物的pH<4时,产物中出现新相磷酸氢钙;当pH=2时,得到纯的磷酸氢钙。
