AIM:To evaluate the clinical effect and complications of two different filling materials(aerocyst urethral catheter and expansion sponges) applying in external dacryocystorhin-ostomy(EXT-DCR) and compare their advantages and disadvantages.METHODS:A retrospective study was made in the period from April,1 2000 to April,1 2005.Totally 180 patients(240 eyes) underwent the EX-DCR using different filling materials and divided into three groups randomly:negative control groups(group 1),expansion sponges group(group 2) and aerocyst urethral catheter group(group 3).The gender,etiology,clinical findings,surgical technique,filling materials,the condition of ocular surface and complications were analyzed.Filling materials were removed during day 7.Postoperative success was determined by lacrimal patency to irrigation,a positive dye test,hemorrhage and errhysis conditions after extubation and subjective resolution of epiphora and liquor puris.RESULTS:During a mean follow-up of 5.14±1.69 years,the success rate were 73.7%(group 1),86.5%(group 2),98.7%(group 3) in three groups.There was significant statistical difference among three groups in the surgical success rate and the operative complications(including hemorrhage,errhysis,periorbital ecchymosis after extubation)(P<0.05).CONCLUSION:EXT-DCR with aerocyst urethral cathete intraoperatively have higher success rate,fewer operative complications and a high patient satisfaction,and can be used to simplify and speed up traditional EXT-DCR.
AIM: To investigate whether the abnormal differentiation of the pterygium epithelium is related to the extracellular signal-regulated kinase(ERK) signaling pathway in vitro.·METHODS: The expression levels of phosphorylated ERK(P-ERK), keratin family members including K19 and K10 and the ocular master control gene Pax-6 were measured in 16 surgically excised pterygium tissues and 12 eye bank conjunctiva. In colony-forming cell assays,the differences in clone morphology and in K10, K19, PERK and Pax-6 expression between the head and body were investigated. When cocultured with the ERK signaling pathway inhibitor PD98059, the changes in clone morphology, colony-forming efficiency,differentiated marker K10, K19 and Pax-6 expression and P-ERK protein expression level were examined by immunoreactivity and Western blot analysis.· RESULTS: The expression of K19 and Pax-6 decreased in the pterygium, especially in the head. No staining of K10 was found in the normal conjunctiva epithelium, but it was found to be expressed in the superficial cells in the head of the pterygium.Characteristic upregulation of P-ERK was observed by immunohistochemistry. The clone from the head with more differentiated cells in the center expressed more K10, and the clone from the body expressed more K19.The P-ERK protein level increased in the pterygium epithelium compared with conjunctiva and decreased when cocultured with PD98059. The same medium with the ERK inhibitor PD98059 was more effective in promoting clonal growth than conventional medium with3T3 murine feeder layers. It was observed that the epithelium clone co-cultured with the inhibitor had decreased K10 expression and increased K19 and Pax-6 expression.· CONCLUSION: We suggest ERK signaling pathway activation might play a role in the pterygium epithelium abnormal differentiation.
·AIM:To develop a new decellularization method depended upon the natural corneal structure and to harvest an ideal scaffold with good biocompatibilities for corneal reconstruction.METHODS:The acellular cornea matrix (ACM) were prepared from de-epithelium fresh porcine corneas (DFPCs) by incubation with 100% fresh human sera and additional electrophoresis at 4℃. Human corneal epithelial cells (HCEs) were used for the cytotoxicity tests of ACM. ACM were implanted into the Enhanced Green Fluorecence Protein (eGFP) transgenic mouse anterior chamber for evaluation of histocompatibility.RESULTS:HE and GSIB4 results showed fresh porcine cornea matrix with 100% human sera and electrophoresis could entirely decellularize stromal cell without reducing its transparency. ACM has no cytotoxic effect ex vivo. Animal test showed there was no rejection for one month after surgery.CONCLUSION:These results provide a decellularizing approach for the study of corneal tissue engineering and had the broader implications for the field of biological tissue engineering in other engineered organ or tissue matrix.
