A three-dimensional micromixer is designed and fabricated by using glass-poly(dimethylsiloxane)(PDMS) hybridized materials.The improvement of the fabrication process makes the micromixer endure much higher flow rate.Based on the self-rotation effect of the fluid,the fast mixing can be achieved.The mixing process is evaluated by connecting the micromixer to a UV-Vis detector.The results show that by adjusting the infuse flow rate,the mixing process can be accurately controlled.