Green fluorescent protein(GFP)gene was inserted into the pseudogene(ψ)region of genome of rabies virus rHep-Flury strain,and a recombinant rabies virus carrying GFP,designated as HEP-GFP,was rescued by reverse genetics system.It was demonstrated that green fluorescent protein could be expressed in the chimeric virus after 5 passages in BHK-21 cell line.The research indicated that the pseudogene(ψ)region in the genome of rHEP-Flury strain,as an independent functional unit in the process of virus assembly,could independently carry and express exogenous genes.
