An amperometric glucose biosensor was developed based on the immobilization of glucose oxidase in the organically modified silicate(ormosil)-polyvinyl acetate(PVA) matrix onto a Prussian Blue(PB)-modified glassy carbon electrode.A higher stability PB-modified electrode was prepared by the electrochemical deposition of FeCl3,K3[Fe(CN)6] and ethylenediamine tetraacetic acid(EDTA) under cyclic voltammetric(CV) conditions.The effects of the potential range of CV conditions,electrolyte cations,applied potential,pH,temperature and co-existing substances were investigated.The detection limit of the glucose biosensor was 8.1 μmol·L-1(S/N = 3) with a linear range from 20 μmol·L-1 to 2 mmol·L-1(R = 0.9965).The biosensor presented a fast response and good selectivity.Additionally,excellent reproducibility and stability of the biosensor were observed.
HaiLing Chen Li Zhao Xi Chen ZhiXia ZhuangXiaoRu Wang
