To obtain transgenic cabbage line with broad insect resistance,a new synthetic Bacillus thuringiensis cry1Ba3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained.Polymerase chain reaction(PCR) and Southern blot analyses confirmed that cry1Ba3 was successfully inserted into the genome of cabbage.Reverse transcription-polymerase chain reaction(RT-PCR) demonstrated that cry1Ba3 was expressed.Western blot results confirmed the production of insecticidal protein encoded by cry1Ba3.Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and Cry1Ac-resistant diamondback moth(DBM) larvae.
YI Deng-xia CUI Lei LIU Yu-mei ZHUANG Mu ZHANG Yang-yong FANG Zhi-yuan YANG Li-mei
