4-Amino-dimethyl-aniline hydrochloride is one of the main components of the wastewater from vanillin production plant. So it is used for the target contamination to be degraded by microorganism, electrochemical oxidation and bio-electro reactor in this paper. We found out a economical, efficient and non-polluted methods for the treatment of wastewater from vanillin production plant. The results indicate that there are a lot of special microbes and bacteria eating 4-amino-dimethyl-aniline hydrochloride in the activated sludge after 20 days′ incubation, at the same time, a stable bio-filmed electrode was formed. Under the condition of 25 ℃, pH=6—7. The use of bio-electro reactor not only increased the biodegradability and decreased the electrical energy consumption, but also solved the problem which the second pollution of organic compounds containing chlorine in the process of indirect electrochemical degradation on the organic pollutants.
The indirect dectrechemical oxidation of 4-amino-dimethyl-aniline hydrochloride containing wastewater generated from vanillin production is presented. Experiments were conducted at a constant current density of 30 rnA/cm^2 via a Ti/Bu-Ti-Sn ternary oxide coated anode and an undivided reactor. During the various stages of the electrolysis, parameters such as the values of chemical oxygen demand（COD） and total organic carbon（TOC） were determined in order to evaluate the feasibility of the electrochemical treatment. The energy consumption and the current efficiency during the electrolysis were calculated. The present study proves the effectiveness of the electrochemical treatment for wastewater resulted from vanillin production.
The immobilization of cytochrome c or horseradish peroxidase at the sulfur-modified gold electrode exhibits a rapid electron transfer behavior because of its specific orientation on the electrode surface and the interaction between cytochrome c or horseradish peroxidase and sulfur-modified on the surface of the Au electrode.
Effect of configuration （structure of electrode, interelectrode gap, positions of inlet and outlet, volume of the cell and additional nets） on mass transfer characteristic of a filter-press type electrochemical cell has been studied. The mass transfer coefficients on the electrodes were obtained by using the well-known technique based on the determination of limiting diffusion current. It is found that mass transfer coefficients with mesh electrode are greater than that of with plate electrode. Mass transfer coefficient is decreased with interelectrode gap. While interelectrode gap achieved a certain value （7 ram）, mass transfer coefficient is steady, no more declining. Mass transfer characteristic for different positions of inlet and outlet are different and dimensionless number groups correlated equations are obtained by experiment. Mass transfer characteristic is the best when inlet located on the top and outlet on the bottom of the cell respectively. While magnified the volume of the cell to eight times, mass transfer characteristic changes little. Mass transfer characteristic without nets is lower than that of with additional nets in the exit region, but higher than that of with additional nets in the entry region.
