Using the optical coherence tomography(OCT) images,the optical attenuation coefficients(μ_t) of human Laogong acupoint and non-acupoint tissues are measured after empty irradiation and 808-nm 100-mW irradiation for 10 min in vivo non-invasively.The results show that there is no significant difference ofμ_t of Laogong acupoint and non-acupoint tissues after empty irradiation.However,there are significant differences ofμ_t between Laogong acupoint and non-acupoint tissues after laser irradiation at the power of 100 mW(statistical definition of probability p0.01).The results of the pilot study indicate that the OCT could distinguish the acupoint from the surrounding tissues after irradiation with laser in vivo non-invasively.