为探究纳米膜和PVC膜对西兰花的保鲜作用,分别采用0.03 mm厚的纳米膜、PVC膜和0.04 mm PE膜(CK)包装西兰花,于常温下贮藏,对其感官、生理指标和营养指标进行测定。结果表明:室温下贮藏,纳米膜和PVC膜包装有效延缓了西兰花外观品质、水分及TSS、V_C、叶绿素等营养物质含量的下降;纳米膜和PVC膜包装可显著抑制西兰花的黄化及MDA积累,增强西兰花POD和CAT活性。这说明纳米膜和PVC膜包装对西兰花的保鲜效果有显著影响,PVC膜包装的西兰花保鲜效果更好。
The antifungal activity of chitosan on a common fungal phytopathogen, Sclerotinia sclerotiorum, and the control effect on sclerotinia rot of carrot were investigated. Mycelial growth and fungal biomass were strongly inhibited by chitosan. Using propidium iodide stain combined with fluorescent microscopy, the plasma membrane of chitosan-treated S. sclerotiorum mycelia was observed to be markedly damaged. Concomitantly, protein leakage and lipid peroxidation was also found to be significantly higher in chitosan-treated mycelia compared to the control. Chitosan provided an effective control of sclerotinia rot of carrot, with induction of activity of defense-related enzymes including polyphenoloxidase and peroxidase. These data suggest that the effects of chitosan on sclerotinia rot of carrot may be associated with the direct damage to the plasma membrane and lipid peroxidation of S. sclerotiorum, and the elicitation of defense response in carrot.