您的位置: 专家智库 > >

国家高技术研究发展计划(2006AA10Z174)

作品数:4 被引量:13H指数:1
相关作者:刘伟华李立会王健胜王辉武军更多>>
相关机构:中国农业科学院作物科学研究所西北农林科技大学更多>>
发文基金:国家高技术研究发展计划国家科技支撑计划更多>>
相关领域:农业科学生物学更多>>

文献类型

  • 4篇中文期刊文章

领域

  • 4篇农业科学
  • 1篇生物学

主题

  • 2篇同源
  • 2篇同源染色体
  • 2篇染色体
  • 2篇基因
  • 2篇PH基因
  • 2篇冰草
  • 2篇P
  • 1篇新种质
  • 1篇穗粒
  • 1篇穗粒数
  • 1篇染色
  • 1篇染色体重组
  • 1篇种质
  • 1篇小麦
  • 1篇粒数
  • 1篇联会
  • 1篇附加系
  • 1篇PROTEO...
  • 1篇2-DE
  • 1篇DEVELO...

机构

  • 2篇中国农业科学...
  • 1篇西北农林科技...

作者

  • 2篇李立会
  • 2篇刘伟华
  • 1篇杨欣明
  • 1篇王睿辉
  • 1篇武军
  • 1篇杨国辉
  • 1篇高爱农
  • 1篇王辉
  • 1篇王健胜

传媒

  • 1篇中国农业科学
  • 1篇科学通报
  • 1篇Scienc...
  • 1篇Journa...

年份

  • 1篇2013
  • 2篇2010
  • 1篇2009
4 条 记 录,以下是 1-4
排序方式:
小麦-冰草多粒新种质及其多粒性遗传分析被引量:12
2009年
【目的】探讨小麦-冰草多粒新种质3228多粒特性(>90粒/穗)的遗传及其利用价值。【方法】将该材料在5个不同小麦种植区进行种植,以评价该材料多粒特性的稳定性;采用主基因+多基因混合遗传模型分析方法对小麦-冰草多粒新种质3228(♀)×京4839(♂)的F2单株表型进行遗传分析。【结果】小麦-冰草多粒新种质3228多粒特性在不同环境下表现稳定,其穗粒数均大于90;小麦-冰草异源新种质3228与京4839(41粒左右/穗)F2群体遗传分析表明,多粒特性主要受1对主效基因控制,主基因的遗传率为27.81%,其加性效应是9.33;此外,在F2群体中,78.11%的单株穗粒数都高于京4839,表明3228中的多粒基因具有一定的显性效应。【结论】小麦-冰草多粒新种质3228多粒特性具有较好的环境稳定性,其在遗传上主要受1对主基因控制,有望利用该多粒基因通过提高穗粒数实现小麦高产育种。
王健胜王辉刘伟华武军李立会
关键词:穗粒数
小麦-冰草附加系1·4重组P染色体对Ph基因的抑制作用被引量:1
2010年
冰草属(AgropyronGaertn.)的P组染色体被推测可能携带有抑制小麦Ph基因的遗传系统,但是相关的研究很少.本研究发现,在小麦-冰草附加系Ⅱ-21-2(附加1·4重组P染色体)的减数分裂中存在染色体联会异常的现象.对该附加系进行细胞遗传学和Ph1基因扩增等分析与检测,结果表明附加系Ⅱ-21-2的Ph1基因扩增正常,未见缺失;小麦-冰草附加系Ⅱ-21-2减数分裂中期每个花粉母细胞出现六价体或四价体的数目分别为0.41和0.13,而附加系受体小麦Fukuho减数分裂无染色体异常联会.双色GISH/FISH检测表明,附加系Ⅱ-21-2的P染色体不直接参与多价体的组成,多价体为小麦自身染色体构成.附加系Ⅱ-21-2的1·4重组P染色体能够抑制小麦Ph基因的作用,从而引起小麦部分同源染色体之间的联会,并造成包括小麦3B-3D等部分同源染色体之间的易位.小麦-冰草附加系的P染色体促进小麦部分同源染色体联会的作用或特性在未来小麦的遗传改良中具有潜在应用价值.
杨国辉杨欣明王睿辉高爱农李立会刘伟华
关键词:PH基因部分同源染色体联会
Comparative Proteomic Analysis of Spike-Development Inhibited and Normal Tillers of Wheat 3558
2013年
Spike number is one of three yield-related factors and is closely related to wheat yield. In the present study, we found that the inhibited and normal tillers of the 3558 line presented phenotypic differences at the elongation stage by morphological and anatomical analysis. We then initiated a proteomic study using two-dimensional electrophoresis (2-DE) and nano- scale liquid chromatography-high-definition tandem mass spectroscopy, to isolate and identify the key proteins and metabolic pathways related to spike-development inhibition. A total of 31 differentially expressed proteins (DEPs), which were mainly involved in cell cycle regulation, photosynthesis, glycolysis, stress response, and oxidation-reduction reactions, were isolated and identified. 14-3-3-1ike proteins and proliferating cell nuclear antigen (PCNA), involved in cell-cycle regulation, were dramatically down-regulated in inhibited tillers compared to normal tillers. Six spots corresponding to degraded Rubisco large subunits, involved in photosynthesis, were detected in different locations of the 2-DE gels and were up-regulated in inhibited tillers. In addition, the relative levels of DEPs involved in glycolysis and oxidation- reduction reactions changed dramatically. Development was blocked or delayed at the elongation stage in the inhibited tillers of 3558. Weakened energy metabolism might be one reason that the inhibited tillers could not joint and develop into spikes. These DEPs and related metabolic pathways are significant for understanding the mechanism of spike-development inhibition and studying the spike-development process in wheat.
ZHENG Yong-shengMA Xiao-gangCHI De-zhaoGAO Ai-nongLI Li-huiLIU Wei-hua
关键词:WHEAT2-DE
The inhibiting effect of 1.4 recombinant P chromosome of wheat-Agropyron cristatum addition line on the Ph gene被引量:1
2010年
P chromosomes may carry a genetic system that inhibits the Ph gene in wheat. Abnormal chromosome synapsis in wheat-Agropyron cristatum addition line II-21-2 (additional 1·4 recombinant P chromosome) was observed in this study. The results of cytogenetics and Ph1 gene amplification showed that the Ph1 gene was normal and the average number of quadrivalents or hexavalents was determined to be 0.41 and 0.13, respectively, in pollen-mother cells of wheat-Agropyron cristatum addition line II-21-2. The analysis of dual-color GISH/FISH showed that the P chromosomes were not directly involved in the composition of multivalents but could inhibit the effect of the Ph gene, leading to synapsis of wheat homoeologous chromosomes and translocation between wheat homoeologous chromosomes such as 3B-3D chromosomes. The characteristic of P chromosomes’ promoting synapsis of wheat homoeologous chromosomes may have potential application in the genetic improvement of wheat.
YANG GuoHui YANG XinMing WANG RuiHui GAO AiNong LI LiHui LIU WeiHua
关键词:染色体重组PH基因附加系冰草同源染色体
共1页<1>
聚类工具0