[Objective] The aim was to investigate the effect of four extraction meth- ods on extraction ratio of 11-α-Hydroxy-Canerenone from biotransformation broth. [Method] Previous reported method, soaking, elution method and homogenate meth- ods were used. [Result] Results showed that more than 90% of the biotransformed 11-α-Hydroxy-Canerenone existed in the broth supernatant and mycelia surface. Elu- tion and soaking methods were effective for 11-α-Hydroxy-Canerenone extraction. Extraction yields of 96.0% and 98.8% were obtained by eluting with 400 ml of ethy- lacetate and soaking with ethylacetate for 90 min, respectively. [Conelusion] The method is simple and efficient, showing industrial application potential.
[Objective] The aim was to study on effect of sludge from different sources on biogas yield efficiency through anaerobic digestion of corn straw. [Method] The present research studied on daily biogas yield and the accumulated biogas amount through anaerobic digestion of corn straw and sludges from four sources. [Result] The accumulated biogas yields produced from sludges in four sources from high to low were granular sludge, river sediments, concentrated sludge and filtered sludge. The first one proved the highest at 3.73 and 56.29 L/kg VS in daily biogas yield and the accumulated biogas. [Conclusion] The research laid foundation for full utilization of straw, improvement of energy utilization and sustainable development.
