This paper described a new method for preparation of ethyl lactate from ammonium lactate directly by means of reactive distillation in the presence of anhydrous aluminium chloride as catalyst and benzene as dehydrating agent.The result showed this method had the advantages of high pure,easy operation,and no pollution.The yield of ethyl lactate reached up to 28.14%,which was 50% higher 50% than that in ordinary reactive device.
Sugar was selected for protecting Candida antarctic lipase B entrapped in sol-gel glass matrix.The activities of immobilized CALB influenced by sucrose,glucose,trehalose,xylose and maltose were investigated.The result showed that trehalose,glucose and xylose were the best protector for lipase CALB.By means of infrared spectrometry and differential scanning calorimeter,the protection mechanism of immobilized CALB by xylose was studied.The results showed that xylose bonded with lipase provided significant improvement in the stability of entrapped CALB.
