Objective To study the characteristic 2-(2-phenylethyl)chromone components of endophytic fungal strain of Aquilaria sinensis by solid culture. Methods The compounds were isolated by various chromatographic methods such as silica gel, reverse-phase silica gel, Sephadex-LH20 column chromatography as well as crystallization. Results Seven 2-(2-phenylethyl)chromone analogues were isolated from the solid culture of Botryosphaeria rhodina A13. Their structures were established by spectral data as well as physicochemical properties, and identified as 6-hydroxy-7-methoxy-2-(2-phenylethyl)chromone(1), 6,7-dimethoxy-2-(2-phenylethyl)chromone(2),(5S,6R,7S,8R)-2-(2-phenylethyl)-5,6,7,8-tetrahydrchromone(3), 6-hydroxy-2-(2-phenylethyl)chromone(4), 4′-hydroxy-2-(2-phenylethyl)chromone(5), 6-methoxy-2-phenethyl-4H-chromen-4-one(6), and 6-methoxy-2-(4′-methoxyphenethyl)-4H-chromen-4-one(7). Conclusion All of the compounds are isolated for the first time from the genus Botryosphaeria. This research opens up a new vista to produce the characteristic components of agarwood by endophytic fungi.
Yao ZhangHong-xin LiuWen-shan LiMei-hua TaoQing-ling PanZhang-hua SunWei YeHao-hua LiWei-min Zhang
