纳米金在生物探针方面的应用技术是重要的研究方向,这一技术的关键在于纳米金与生物分子有效的结合,本文通过基因重组策略,研究杆状病毒表面展示技术与金特异结合肽介导的固定化方法联用以构建生物-无机复合材料的可行性。利用Bac-to-Bac杆状病毒表达系统将合成的金特异结合肽基因融合到杆状病毒BmNPV囊膜糖蛋白gp64的N端信号肽与成熟蛋白之间构成转移质粒载体,经过转座得到重组穿梭质粒pFB-gp64-Au,转染Sf9昆虫细胞后收获多角体启动子控制下表达融合蛋白的重组AcMNPV。同时采用硼氢化钠还原法制备得到胶体金,进行组建病毒-胶体金复合结构的初步研究。结果表明,成功构建的重组穿梭质粒转染细胞后得到囊膜表面经金无机结合肽修饰的AcMNPV重组病毒,并通过透射电子显微镜(transmission electron microscopy,TEM)观察到由金特异结合肽所介导的病毒-胶体金的复合结构。这项研究有助于进行生物-无机复合体系构建及纳米材料在生物学领域应用的研究。
Low molecular weight poly(glycidyl methacrylate)s (PGMAs) were prepared by photopolymerization in ethyl acetate, with benzophenone (BP) as photoinitiator, and triethylamine (TEA) as hydrogen donor. The existence of semipinacol dormant end groups in PGMA was confirmed by FT-IR and 1H-NMR, and the content of the semipinacol dormant groups was determined quantitatively by 1H-NMR measurement. The effects of various factors, such as reaction time, BP concentration and monomer concentration on the synthesis of the polymers were investigated systematically. The molecular weights of the polymers were also investigated with GPC. It is shown that increasing BP concentration and decreasing irradiation time and monomer concentration led to a significant decrease of the molecular weights.
