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国家自然科学基金(30340420442)

作品数:2 被引量:0H指数:0
相关作者:王世珍许淼黄萱孙占华隋森芳更多>>
相关机构:清华大学更多>>
发文基金:国家自然科学基金国家重点基础研究发展计划更多>>
相关领域:生物学更多>>

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Designing the lipid raft marker protein for synaptic vesicles
2009年
Lipid rafts are cholesterol-enriched microdomains and implicated in many essential physiological ac-tivities such as the neurotransmitter release.Many studies have been carried out on the function of rafts inthe plasma membranes,whereas little is known about the information of such microdomains in subcellularcompartments especially synaptic vesicles(SVs).In the well-studied plasma membranes,several proteinshave been recognized as raft markers,which are used to label or trace rafts.But the raft marker proteinon SVs has not been identified yet.Although some SV proteins,including VAMP and CPE,have beenfound in raft fractions,they cannot be used as markers due to their low abundance in rafts.In this work,we designed several chimera proteins and tested their characteristics for using as SV raft makers.First,we detected whether they located in SVs,and then the chimeras exhibiting the better localization in SVswere further examined for their enrichment in raft using detergent treatment and gradient density floatationanalysis.Our results indicate that one of the chimeric proteins is primarily located in SVs and distributedin raft microdomains,which strongly suggests that it could be served as a raft marker for SVs.
吕继华SuiSenfang
镁离子转运蛋白CorA间质结构域删除分析
2008年
为了研究CorA的镁离子转运机理,对大肠杆菌CorA的间质结构域区进行了删除突变分析,并利用酵母突变体互补技术进行了突变体活性测定。以酿酒酵母质膜镁离子转运系统敲除突变体菌株CM66及其基因型对照菌株CM52为宿主菌,建立了大肠杆菌CorA转运活性测定体系。结果显示:大肠杆菌CorA间质结构域N-端起始的24个残基对于CorA在酵母质膜中的表达或维持其本身正确构象发挥重要作用,CorA M124到D154段的部分残基在CorA介导的镁离子转运过程中起到重要作用。
孙占华王世珍黄萱许淼隋森芳
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