Hyphantria cuea nucleopolyhedrovirus(HcNPV) and several other nucleopolyhedrosis was extracted by differential centrifugation,and purified with sucrose gradient centrifugation method.Polyhedrin was gotten and analysed by SDS-PAGE.It indicated that most of those proteins had 32 KD protein band,and with 64 KD protein,and might be the main protein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virus protein in 18 KD department had a specific band.
The combinations of Hyphantria cunea NPV(HcNPV) and three different fluorescent brighteners BA,VBL,CBS-X were added to the artificial diet and the influence of synergists in concentration 25 ℃ and 14L/10D(h).The results showed that the fluorescent brighteners BA and VBL had higher insecticidal activity,CBS-X had not significant synergistic effect.
Bioassay of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon was conducted on 2-instar larvae of Parocneria orientia in lab.The results showed that 1%VBL,12.5 μL·L-1 Chlorbenzuron,15 μL·L-1 Trichlorphon had better synergistic effect.Spraying of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon and PoNPV to control 2～3 instar larvae of second generation in field,the concentrations of PoNPV were 8×106 PIB·mL-1,4×106 PIB·mL-1,2×106 PIB·mL-1,1×106 PIB·mL-1,5×105 PIB·mL-1,there were 20 treatments.It was indicated that when the concentration was the same,the effect of PoNPV added synergists was better than that without synergists.
