Low molecular weight polypeptide was gotten by microwave heating and two kinds of enzyme hydrolysis.It was tested and seperated by high-performance capillary electrophoresis(HPCE),gel permeation chromatography(GPC).The results showed that microwave heating and enzyme hydrolysis was a high effect way of hydrolysis protein into low molecular weight polypeptide;polypeptide was well tested and seperated by HPCE,consociation G-50 with gel permeation chromatography(GPC).