随着环境污染日益严重,对环境污染评价的研究亦愈发深入。基于核磁共振(Nuclear Magnetic Resonance,NMR)代谢组学是近年发展起来的一种新型环境污染评价方法,它是以核磁共振为技术手段,从生物角度研究生物体暴露在环境污染物下,生物体内源性代谢产物谱的变化,以期预警环境污染的程度。本文将综述基于核磁共振的代谢组学在环境污染评价上的原理、研究思路、以及国内外研究新进展。
Crab paste is a traditional and popular food for people in the coastal area of China.Visual inspection is currently the only method of quality assessment.We developed a new analytical method of a comprehensive survey of nutrients and quality assessment for crab paste based on a combination of 1 H NMR spectroscopy with multivariate data analysis.The aqueous extract of crab paste was dominated by amino acids,sugars,carboxylic acids,nucleotides and amines(including 19 first-time-reported compounds such as choline,uracil and guanosine).Two grades of crab paste had significant compositional differences in terms of amino acids,lactate,N-acetylglutamate,choline,dimethylamine,uridine,1-methylnicotinamide and 2-pyridienmethanol.These results provided important information on the grade-dependence of crab-paste composition,and demonstrated that NMR-MDA was effective not only for the comprehensive survey of nutrients,but also for quality assessment of crab paste.
