We studied the protective effect of xylocoside G on Aβ25-35-induced apoptosis in PC12 cells. Cell viability was analyzed by MTT assay, and apoptotic neuronal death was assessed by Hoechst 33342 staining. Flow cytometry was used to determine the apoptotie neuronal cells quantitatively. The level of intracellular reactive oxygen species （ROS） was monitored with the fluorescent probe 2＇,7＇-dichlorofluoresce in diacetate （DCFH-DA）. We found that PC12 cells treated with 25 pM Aβ25-35 for 24 h had a significant decrease in cell viability compared with control, and the percentage of apoptotic cells was increased to 34.26%. The level of intracellular oxygen species was also increased. Co-incubation with xylocoside G （10μM） for 24 h attenuated the damaging effect of Aβ25-5 on the cell viability, and the percentage of apoptotic cells was decreased to 22.62%. Moreover, the increase of ROS induced by Aβ25-5 was inhibited by xylocoside G （10μM）. We concluded that xylocoside G had protective effect against Aβ25-35-induced apoptosis, which might be related with the decrease of the level of ROS.
目的探讨轻度认知损害(M C I)患者认知功能改变以及认知功能改变和载脂蛋白E(A poE)ε4等位基因的关系。方法应用神经心理学方法评估患者的认知功能状况,应用分子生物学的方法进行APOE的基因型检测。结果①除记忆力显著下降外,M C I组定向力、语言能力、执行等能力也呈下降趋势。②M C I组ε4等位基因出现的频率远高于正常老年组(P<0.01)。③A poEε4的携带者的认知功能的总评分显著低于非A poEε4携带者(P<0.001)。除记忆力表现出显著下降外,A poEε4携带组的语言能力、执行也呈下降趋势(P<0.05)。结论轻度认知功能损害患者的认知功能出现改变,且A poEε4等位基因出现的频率显著高于正常老年组。A poEε4等位基因与老年人认知功能的下降相关。
