In order to get an acceptable interests-distribution scheme for each partner of enterprise-university-research institute cooperation in advance,based on Nash model,the paper designs the interest distribution method of enterprise-university-research institute cooperation and provides the basis for determining the weight of each partner in accordance with the interest distribution principles and subjected relations between major factors in enterprise-university-research institute cooperation.Also,in combination with one example,an applicable method is provided to distribute interest in enterprise-university-research institute cooperation.The study gives some references for interests-distribution of enterprise-university-research institute cooperation.