In plants, non-coding small RNAs play a vital role in plant development and stress responses. To explore the possible role of non-coding small RNAs in the regulation of the jasmonate （JA） pathway, we compared the non-coding small RNAs between the JA-deficient aos mutant and the JA-treated wild type Arabidopsis via high-throughput sequencing. Thirty new miRNAs and 27 new miRNA candidates were identified through bioinformatics approach. Forty-nine known miRNAs （belonging to 24 families）, 15 new miRNAs and new miRNA candidates （belonging to 11 families） and 3 tasiRNA families were induced by JA, whereas 1 new miRNA, 1 tasiRNA family and 22 known miRNAs （belonging to 9 families） were repressed by JA.
Jasmonate （JA） regulates various plant defense and developmental processes. The F-box protein CORONATINE INSENSITIVE 1 （COIl） perceives JA signals to mediate diverse plant responses including male fertility, root growth, antho- cyanin accumulation, and defense against abiotic and biotic stresses. In this study, we carried out genetic, physiological and biochemical analysis on a series of coil mutant alleles, and found that different amino acid mutations in COIl distinctively af- fect JA-regulated male fertility in Arabidopsis. All the JA responses are disrupted by the COIl mutations W467＂ in coil-l, Q343＂ （coil-6）, G369E （coil-4）, G98D （coil-5）, G155E （coil-7）, D452A （coil-9） and L490A （coil-10）, though the coil-5 mutant （COIlG98D） contains adequate COIl protein （~60% of wild-type）. Interestingly, the low basal level of COIlE543K in the coil-8 mutant （-10% of wild-type COIl level） is sufficient for maintaining male fertility （-50% of wild-type fertility）; the coil-2 mutant with low level of COIlLz45F （-10% of wild-type） is male sterile under normal growth condition （22℃） but male fertile （~80% of wild-type fertility） at low temperature （16℃）; however, both coil-2 and coil-8 are defective in the other JA responses （root growth, anthocyanin accumulation, and plant response to the pathogen Pst DC3000 infection）.
HUANG Huang WANG CuiLi TIAN HaiXia SUN Yu XIE DaoXin SONG SuSheng
